荧光染料光谱列表

荧光染料光谱列表 染料名称Excitation(nm)Emission(nm)分子量备注信息 Reactive and conjugated probes Hydroxycoumarin325386 3 31 3 30 3 17 5 96 4 06 Succinimidyl ester Aminocoumarin350445Succinimidyl ester Methoxycoumarin360410Succinimidyl ester Cascade Blue(375);401423Hydrazide Pacific Blue Pacific Orange Lucifer yellow NBD R-Phycoerythrin (PE) PE-Cy5 conjugates 403 403 425 466 455 551 528 539 Maleimide 2 94 2 40 k NBD-X 480;565 480;565;6 50 480;565;7 43 480;565 490 490,675 494 578 670 aka Cychrome, R670, Tri-Color, Quantum Red PE-Texas Red Peridinin chlorphyll protein PerCP-Cy5.5 conjugate 5 87 3 89 GE Healthcare PE-Cy7 conjugates Red 613 PerCP TruRed FluorX 767 613 675 695 520 Fluorescein BODIPY-FL TRITC 495 503 547 519 512 572 FITC; pH sensitive 4 44 5 48 TRITC X-Rhodamine Lissamine Rhodamine B 570576XRITC 570590 Texas Red Allophycocyanin (APC) APC-Cy7 conjugates 589615 6 25 1 04 k Sulfonyl chloride 650660 650;755767PharRed Alexa Fluor 系列荧光染料 Alexa Fluor 350343442 4 10 1 028 7 02 6 43 7 00 7 14 7 24 1 079 1 250 7 92 8 20 1 285 1 200 1 300 1 100 Alexa Fluor 405401421 Alexa Fluor 430434540 Alexa Fluor 488499519QY 0.92 Alexa Fluor 500503525 Alexa Fluor 514517542 Alexa Fluor 532530555QY 0.61 Alexa Fluor 546561572QY 0.79 Alexa Fluor 555553568QY 0.1 Alexa Fluor 568579603QY 0.69 Alexa Fluor 594591618QY 0.66 Alexa Fluor 610610629 Alexa Fluor 633632648 Alexa Fluor 647652668QY 0.33 Alexa Fluor 660 Alexa Fluor 680 663 680 691 7021 150 Alexa Fluor 700696719 1 400 1 300 1 750 Alexa Fluor 750752776 Alexa Fluor 790 Cy 系列荧光染料 Cy2 782804 489506 570;(615 ) 572;(620 ) 594;(640 ) 670 7 14 7 67 6 58 1 102 7 92 1 128 8 18 QY 0.12 Cy3(512);550QY 0.15 Cy3B558QY 0.67 Cy3.5581QY 0.15 Cy5(625);650QY 0.28 Cy5.5675694QY 0.23 Cy7 核酸相关探针染料 Hoechst 33342 DAPI Hoechst 33258 743767QY 0.28 343 345 345 483 455 478 6 16 AT-selective AT-selective 6 24 ~ 400 AT-selective SYTOX Blue Chromomycin A3 Mithramycin YOYO-1 431 445 445 491 480 575 575 509 DNA CG-selective 1 271 3 94 Ethidium Bromide Acridine Orange SYTOX Green 493 503 504 620 530/640 523 DNA/RNA ~ DNA 600 Vital stain, TOTO: Cyanine Dimer TOTO-1, TO-PRO-1509533 TO-PRO: Cyanine Monomer Thiazole Orange Propidium Iodide (PI) LDS 751 7-AAD SYTOX Orange TOTO-3, TO-PRO-3 DRAQ5 细胞功能探针 Indo-1361/330490/405 1 010 8 55 AM ester. Low/High Ca++, 510 536 530 617 6 68.4 4 72 543;590 546 547 642 647 712;607 647 570 661 681,697 DNA (543ex/712em), RNA (590ex/607em) 7-aminoactinomycin D, CG-selective ~ 500 DNA 4 13 usable excitation down to 488 Fluo-3506526AM ester. pH 6 DCFH505535 5 2 7 Dichorodihydrofluorescein, 29 3 46 oxidized Dihydrorhodamine 123, oxidized , light catalyzes oxidation pH 6/9 DHR505534 SNARF 荧光蛋白 Y66H Y66F EBFP 548/579587/635 360 360 380 442 508 440 QY .18 BR 0 .27 0 5 2 PS 0 monomer EBFP2383448monomer Azurite GFPuv 383 385 447 508 1 monomer T-Sapphire399511 .60 .62 .40 .15 .51 .24 0 6 0 7 0 3 0 2 5 2 6 1 4 3 1 89 3 2 2 weak dimer Cerulean433475 3 weak dimer mCFP433475 6 monomer ECFP434477 5 CyPet Y66W mKeima-Red 435 436 440 477 485 620