AM激活mTOR信号通路对人卵巢癌细胞增殖的影响的开题报告

精品文档---下载后可任意编辑 AM激活mTOR信号通路对人卵巢癌细胞增殖的影响的开题报告 Introduction Ovarian cancer is the fifth most common cancer in women worldwide and is one of the leading causes of cancer-related deaths in women. The treatment for ovarian cancer involves a combination of surgery and chemotherapy; however, the prognosis for patients with advanced stage ovarian cancer remains poor. Therefore, there is a need for new interventions that can effectively target ovarian cancer cells. The mammalian target of rapamycin mTOR is a protein kinase that plays a crucial role in cell growth, proliferation, and survival. Dysregulated mTOR signaling has been associated with numerous diseases, including cancer. Activated mTOR signaling promotes cancer cell growth and survival, making it an attractive target for cancer therapy. In recent years, several studies have shown that activating the mTOR signaling pathway can promote the proliferation of ovarian cancer cells. However, the exact mechanisms underlying this phenomenon remain unclear. Therefore, this study aimed to investigate the impact of AM activation of the mTOR signaling pathway on the proliferation of human ovarian cancer cells. Hypothesis Activation of the mTOR signaling pathway by AM promotes the proliferation of human ovarian cancer cells. Objectives The primary objective of this study is to investigate the effect of AM activation of the mTOR signaling pathway on the proliferation of human ovarian cancer cells. The secondary objectives include 1. To investigate the effect of AM on the levels of key proteins involved in the mTOR signaling pathway. 2. To uate the expression levels of the AM receptor in human ovarian cancer cells. 3. To investigate the effect of mTOR pathway inhibition on the proliferation of human ovarian cancer cells. s Cell culture Human ovarian cancer cell lines A2780 and SKOV3 will be cultured in RPMI-1640 medium supplemented with 10 fetal bovine serum FBS and 1 penicillin and streptomycin. Cells will be incubated at 37C in a humidified atmosphere containing 5 CO2. Cell viability assay To investigate the effect of AM activation of the mTOR signaling pathway on the proliferation of human ovarian cancer cells, a cell viability assay will be pered using the MTT assay. Cells will be plated in a 96-well plate and treated with AM or rapamycin mTOR inhibitor for 24 and 48 hours. MTT assays will be used to measure the cell viability. Western blot analysis To investigate the effect of AM on the levels of key proteins involved in the mTOR signaling pathway, western blot analysis will be pered. Cells will be treated with AM for 24 hours. The expression levels of mTOR, p-mTOR, p70S6K, and p-P70S6K will be analyzed by western blotting. Real-time PCR To uate the expression levels of the AM receptor in human ovarian cancer cells, real-time PCR will be conducted. Total RNA will be isolated from cells using TRIzol reagent. cDNA will be synthesized using the High-Cap