医学毕业论文胎儿心脏黏附细胞具有类似间充质祖细胞特征

XX大学 毕业论文 胎儿心脏黏附细胞具有类似间充质祖细胞特征 2014年6月25日 胎儿心脏黏附细胞具有类似间充质祖细胞特征 作者江小霞，苏永锋，李秀森，张毅，吴英，毛宁 【摘要】为了观察人心脏是否含具有间充质祖细胞特性的细胞，从胎儿 心脏分离、培养单个核细胞并从形态、表型和功能3个方面与骨髓间充质祖细胞 进行比较和鉴定。结果表明，从心脏分离培养的细胞为成纤维样，表面抗原为 CD73, CD105, CD29, CD44, HLA-ABC, CD 166 阳性，而 CD45, CD34, CD86, HLA-DR阴性。在不同的分化体系中，细胞能分化为脂肪细胞、成骨细胞和软 骨细胞。细胞扩增迅速，具有低免疫原性特性。结论从心脏分离培养的细胞具 有间充质祖细胞特性。 【关键词】胎儿心脏；心脏黏附细胞；间充质祖细胞 Human Fetal Heart-derived Adherent Cells with Characteristics Similar to Mesenchymal Progenitor Cells AbstractThis study was aimed to investigate if human heart harbored a population of primitive undifferentiated cells with the characteristics of MPC. Cells were isolated from human fetal heart and were cultured under conditions appropriate for bone marrow-derived MPCs. Their morphology, phenotypes and functions were tested by s developed for MPC from other sources. The results showed that morphologically, cells were spindle shaped and resembled fibroblasts. In their undifferentiated state, cells were CD73, CD105, CD29, CD44, HLA-ABC, CD 166 positive and CD45, CD34, CD86, HLA-DR negative. When cultured in adipogenic, osteogenic or chondrogenic media, cells differentiated into adipocytes, osteocytes and chondrocytes respectively. They could be extensively expanded in vitro and exhibited very low immunogenicity as uated by T cell proliferation assays. It is concluded that cells isolated from fetal heart possess simi-larity to their adult and fetal bone marrow counterparts in morphologic, immunophenotypic, and functional characteristics. Key wordsfetal heart; heart derived adherent cell; mesenchymal progenitor cell Bone marrow-derived mesenchymal progenitor cells MPC have attracted great attention because of their capability for renewal and differentiation into various lineages of mesenchymal tissues [ 1 ] and their potential plats for the systemic delivery of therapeutic proteins in vivo following gene transfer using oncogenic retroviruses [2] . Studies involving a variety of animal models have shown that adult bone marrow-derived MPC can migrate and engraft in numerous organs and differentiate along tissue-specific lineages under the stimulation of local factors, and may be useful in the repair or regeneration of damaged or mutated bone, cartilage, or myocardial tissues [3-5] .Recent work has shown that MPC are present in many tissues, including umbilical cord [6] , umbilical cord blood [7] , bone marrow, fetal blood, and fetal li-ver [8] . Though the use of MPC in the treatment of acute myocardial infarction has become a novel therapeutic option, the knowledge of their presence in heart is limited [ 9 ] . Our aim was to investigate whether there were cells with the characteristics of MPC in human fetal heart. Materials and s Isolation and culture of adult and fetal bone mar-row, fetal heart mesenchymal progenitor cells The Research Ethics Committees of Xuanwu Hospital approved human tissue for research purposes.Human fetal heart samples were obtained fr